Lima Cheol-Hong, Yu Il Je, Kim Hyeon-Young, Lee Seung-Bae, Marshak Daniel R, Lee Ji Hyun, Kim Kwang Jong
Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Saftty and Health Agency, Daejeon, South Korea.
Toxicol Ind Health. 2005 Oct;21(9):207-13. doi: 10.1191/0748233705th230oa.
Three groups of male F344 rats were exposed to a water-soluble metal working fluid (MWF) aerosol at concentrations of 20, 60 or 180 mg/m3 for 6 h/day, five days a week, for 13 weeks in inhalation chambers. The aerosol particles were normally distributed and the mass median aerodynamic diameter was 1.56 microm. Despite the absence of clinical findings or significant changes in body weight during the 13-week exposure period, the numbers of white blood cells and lung weights were significantly higher at the end of the 13-week exposure period. Exposure to 20 mg/m3 of the MWF aerosol was found to have an effect on the respiratory system, including an accumulation of foamy macrophages in the bronchoalveolar lavage (BAL) fluid and thickening of the alveolar walls in the histopathology. The level of histamine and number of polymorphonuclear (PMN) cells were also higher in the BAL fluid from the rats exposed to 60 mg/m3 of the MWF aerosol, while the respiratory inflammation was most pronounced in the rats exposed to 180 mg/m3 of the MWF aerosol, including the accumulation of PMNs and foamy macrophages in the BAL cells, lung weight increase and thickening of the alveolar walls. Immunoglobulin IgG2a level was also lower in the sera from the rats exposed to 180 mg/m3 of the MWF aerosol. Therefore, even though no clinical symptoms were observed in the rats exposed to the high MWF concentration, respiratory inflammation was still induced by a relatively low concentration of the MWF, while the immune system was affected by the high MWF concentration.
将三组雄性F344大鼠置于吸入舱中，使其每天6小时、每周5天暴露于浓度为20、60或180毫克/立方米的水溶性金属加工液（MWF）气溶胶中，持续13周。气溶胶颗粒呈正态分布，质量中位空气动力学直径为1.56微米。尽管在13周的暴露期内未观察到临床症状或体重有显著变化，但在13周暴露期结束时，白细胞数量和肺重量显著增加。发现暴露于20毫克/立方米的MWF气溶胶对呼吸系统有影响，包括支气管肺泡灌洗（BAL）液中泡沫巨噬细胞的积聚以及组织病理学上肺泡壁的增厚。暴露于60毫克/立方米MWF气溶胶的大鼠的BAL液中组胺水平和多形核（PMN）细胞数量也更高，而暴露于180毫克/立方米MWF气溶胶的大鼠的呼吸道炎症最为明显，包括BAL细胞中PMN和泡沫巨噬细胞的积聚、肺重量增加以及肺泡壁增厚。暴露于180毫克/立方米MWF气溶胶的大鼠血清中的免疫球蛋白IgG2a水平也较低。因此，即使在暴露于高MWF浓度的大鼠中未观察到临床症状，但相对低浓度的MWF仍可诱发呼吸道炎症，而高MWF浓度会影响免疫系统。
