Ding Jian, Yu Jieping, Wang Chengdang, Hu Wei, Li Dan, Luo Yanjun, Luo Hesheng, Yu Honggang
Digestive Department of the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Liver Int. 2005 Dec;25(6):1224-32. doi: 10.1111/j.1478-3231.2005.01169.x.
To investigate the protective effect of Ginkgo biloba extract (GbE) on liver fibrosis induced by carbon tetrachloride (CCl4) in rats and expressions of transforming growth factor beta1 (TGF-beta1) and collagen I during this period.
The effect of GbE on liver fibrogenesis was detected by hematoxylin and eosin staining (H&E staining), Masson's trichrome staining, and electron microscope study. Blood samples were collected for measurement of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin. Malondialdehyde (MDA) in liver tissue was detected by the thiobarbituric acid (TBA) method. Immunohistochemistry assay and RT-PCR were used to examine the protein expressions and mRNA levels of TGF-beta1 and collagen I, respectively.
H&E, Masson's trichrome stainings and electron microscope study showed liver fibrosis in rats was greatly alleviated when treated with GbE. Additionally, there was a remarkable improvement of serum ALT, AST, albumin and MDA in the GbE-treated group. Immunohistochemistry and RT-PCR results showed GbE intervention significantly inhibited TGF-beta1 and collagen I expressions in rat liver. No side effects of GbE were found during these experiments. But GbE could not reverse the pathological changes of liver fibrosis completely when compared with normal control.
GbE can partially protect rat liver from the fibrogenesis induced by CCl4. The mechanism may lie in its effect of inhibiting oxidative stress caused by liver injury and expressions of signal molecules such as TGF-beta1. GbE may thus be of potential help as a medicament or food additive for alleviation of liver fibrogenesis.
研究银杏叶提取物（GbE）对四氯化碳（CCl4）诱导的大鼠肝纤维化的保护作用以及在此期间转化生长因子β1（TGF-β1）和Ⅰ型胶原的表达。
通过苏木精-伊红染色（H&E染色）、Masson三色染色和电子显微镜研究检测GbE对肝纤维化形成的影响。采集血液样本测定丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和白蛋白。采用硫代巴比妥酸（TBA）法检测肝组织中的丙二醛（MDA）。分别用免疫组织化学分析和RT-PCR检测TGF-β1和Ⅰ型胶原的蛋白表达和mRNA水平。
H&E染色、Masson三色染色和电子显微镜研究显示，GbE治疗可使大鼠肝纤维化得到显著缓解。此外，GbE治疗组的血清ALT、AST、白蛋白和MDA有明显改善。免疫组织化学和RT-PCR结果显示，GbE干预可显著抑制大鼠肝脏中TGF-β1和Ⅰ型胶原的表达。在这些实验中未发现GbE有副作用。但与正常对照组相比，GbE不能完全逆转肝纤维化的病理变化。
GbE可部分保护大鼠肝脏免受CCl4诱导的纤维化。其机制可能在于其抑制肝损伤引起的氧化应激以及TGF-β1等信号分子表达的作用。因此，GbE作为一种药物或食品添加剂，在减轻肝纤维化方面可能具有潜在的帮助。
