Dos Santos Jair Guilherme, Longo Beatriz Monteiro, Blanco Mirian Marcela, Menezes de Oliveira Maria Gabriela, Mello Luíz Eugênio
Department of Psychobiology, Universidade Federal de São Paulo, R. Botucatu 862, 5 andar, 04023-062 São Paulo, Brazil.
Brain Res. 2005 Dec 20;1066(1-2):37-48. doi: 10.1016/j.brainres.2005.09.037.
Cycloheximide influences synaptic reorganization resulting from pilocarpine-induced status epilepticus (SE). To investigate the possible behavioral consequences of this effect, we subjected animals to pilocarpine-induced SE either in the absence (Pilo group) or presence of cycloheximide (Chx group). Animals were further divided regarding the occurrence of spontaneous recurrent seizures (SRS). Two months after SE induction animals were exposed to different behavioral tests. Age-matched naïve animals were used as controls. All epileptic groups showed a significantly diminished freezing time in contextual and tone fear conditioning, performed poorly in the Morris water maze and present less seconds in immobility position as compared to controls. Only Pilo animals explored more extensively the open arms of the elevated plus maze and showed increased in horizontal exploratory activity in the open field as compared to controls. With the exception of Pilo animals without recorded SRS, all other groups had extensive tissue shrinkage in central nucleus of the amygdala as compared to controls. Cycloheximide-treated animals differed from Pilo animals in the extent of hilar loss and supragranular mossy fiber sprouting as well as tissue shrinkage in the dorsal hippocampus. Despite the histological differences seen in the dorsal hippocampus between experimental groups, no differences were encountered in the cognitive tests used to evaluate dorsal hippocampal function. The encountered histological differences between Chx and Pilo animals, however, might underlie the different emotional responses between the two groups.
放线菌酮会影响毛果芸香碱诱导的癫痫持续状态（SE）所导致的突触重组。为了研究这种效应可能产生的行为后果，我们使动物在不存在放线菌酮（毛果芸香碱组）或存在放线菌酮（放线菌酮组）的情况下经历毛果芸香碱诱导的SE。动物还根据自发性复发性癫痫发作（SRS）的发生情况进一步分组。SE诱导两个月后，让动物接受不同的行为测试。将年龄匹配的未经历过实验的动物用作对照。与对照组相比，所有癫痫组在情境和音调恐惧条件反射中的僵住时间均显著减少，在莫里斯水迷宫中表现不佳，在静止位置的时间也更少。与对照组相比，只有毛果芸香碱组的动物更广泛地探索高架十字迷宫的开放臂，并且在旷场试验中的水平探索活动增加。与对照组相比，除了未记录到SRS的毛果芸香碱组动物外，所有其他组的杏仁核中央核均有广泛的组织萎缩。放线菌酮处理的动物在海马门区损失程度、颗粒上层苔藓纤维出芽以及背侧海马组织萎缩方面与毛果芸香碱组动物不同。尽管实验组之间在背侧海马观察到组织学差异，但在用于评估背侧海马功能的认知测试中未发现差异。然而，放线菌酮组和毛果芸香碱组动物之间观察到的组织学差异可能是两组之间不同情绪反应的基础。
