Chardin H, Gokani J P, Septier D, Ruch J V, Goldberg M
Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, Montrouge, France.
Histochem J. 1992 Jun;24(6):375-82. doi: 10.1007/BF01046170.
The ultrastructural appearance of different types of basement membrane was studied using histochemical methods for visualizing glycosaminoglycans. Samples of rat gingiva and mouse molar germ tissue were fixed either with glutaraldehyde, glutaraldehyde-ruthenium hexammine trichloride (RHT), glutaraldehyde-Cuprolinic Blue (CB) or cetylpyridinium chloride-glutaraldehyde (CPC). Ultrathin sections were stained with uranyl acetate and lead citrate. The results showed that the conventional trilaminar structure of the basement membrane was observed after glutaraldehyde and CB fixation. In contrast, after CPC or RHT fixation, the appearance of the basement membrane was homogeneous without any evidence of a lamina lucida. Furthermore, after single fixation with CPC, the ultrastructure of different basement membranes from oral tissues showed some differences in appearance which were related to their localizations, functions, or both.
使用组织化学方法可视化糖胺聚糖，研究了不同类型基底膜的超微结构外观。用戊二醛、戊二醛-三氯化六氨合钌（RHT）、戊二醛-铜试剂蓝（CB）或十六烷基吡啶氯化物-戊二醛（CPC）固定大鼠牙龈和小鼠磨牙胚组织样本。超薄切片用醋酸铀和柠檬酸铅染色。结果表明，戊二醛和CB固定后观察到基底膜的传统三层结构。相比之下，CPC或RHT固定后，基底膜外观均匀，没有任何透明板的迹象。此外，用CPC单次固定后，口腔组织不同基底膜的超微结构在外观上显示出一些差异，这些差异与其定位、功能或两者有关。
