Boukari A, Ruch J V
J Biol Buccale. 1981 Dec;9(4):349-61.
First mandibular embryonic mouse molars (day 18) were cultured for 8 days. Maintenance of crown pattern and amelogenesis were studied in function of different culture conditions. Grown on top of semi-solid coagulum (composed of cock plasma, embryonic extract, fetal calf serum and MEM or BGjb) the typical crown pattern was always conserved. Amelogenesis existed in 85% if the coagulum was composed of 60% BGjb, 30% plasma, 10% embryonic extract and 180 microgram/ml ascorbic acid. Embryonic extract did not inhibit the mineralization. Grown on Millipore filter in the presence of different media (MEM or BGJb supplemented with serum, embryonic extract and ascorbic acid) the crown pattern was always disturbed. Nevertheless amelogenesis was generally initiated. In absence of serum and embryonic extract, chemically defined media (MEM or BGjb, supplemented with glutamine, glycine and ascorbic acid) allowed functional differentiation of odontoblasts and polarization of ameloblasts. These cells did not secrete enamel. If these defined media were devoid of ascorbic acid, predentin was secreted but necrosis of dental papilla cells was observed.
将第18天的小鼠下颌第一磨牙胚胎培养8天。根据不同的培养条件研究牙冠形态的维持和成釉作用。生长在半固体凝块（由公鸡血浆、胚胎提取物、胎牛血清和MEM或BGjb组成）上时，典型的牙冠形态总是得以保留。如果凝块由60%的BGjb、30%的血浆、10%的胚胎提取物和180微克/毫升的抗坏血酸组成，85%的样本存在成釉作用。胚胎提取物不抑制矿化。生长在密理博滤膜上且存在不同培养基（补充了血清、胚胎提取物和抗坏血酸的MEM或BGJb）时，牙冠形态总是受到干扰。然而，成釉作用通常会启动。在没有血清和胚胎提取物的情况下，化学成分确定的培养基（补充了谷氨酰胺、甘氨酸和抗坏血酸的MEM或BGjb）能使成牙本质细胞发生功能分化，成釉细胞发生极化。这些细胞不分泌釉质。如果这些确定的培养基不含抗坏血酸，会分泌前期牙本质，但会观察到牙乳头细胞坏死。
