Freshwater Biological Association, Far Sawrey, Ambleside, Cumbria, U.K.
Appl Environ Microbiol. 1982 Mar;43(3):542-7. doi: 10.1128/aem.43.3.542-547.1982.
Three distinct phases were observed in the change of dissolved inorganic nitrogen concentrations in the hypolimnion of Grasmere. The second phase of decreasing ammonia and increasing nitrate concentrations was typical of the nitrification process. Observations on nitrate concentration gradients between surface sediments and the water column and experiments using the nitrification inhibitor N-Serve indicated the in situ activity of chemolithotrophic nitrifying organisms. Nitrification rates were estimated throughout the period of stratification by using the N-Serve and [C]bicarbonate uptake method. Comparison of the field nitrate concentrations with the predicted nitrate concentrations (from estimates of the nitrification rate) indicated that the method underestimated the true rate of nitrification. Possible reasons for this are discussed.
在格拉斯米尔湖底层溶解无机氮浓度的变化过程中观察到三个明显的阶段。氨浓度降低和硝酸盐浓度增加的第二阶段是硝化过程的典型特征。对表层沉积物和水柱之间硝酸盐浓度梯度的观察以及使用硝化抑制剂 N-Serve 的实验表明，化能自养硝化生物在现场具有活性。在分层期间，通过使用 N-Serve 和[C]碳酸氢盐摄取方法来估计硝化速率。将现场硝酸盐浓度与预测硝酸盐浓度（根据硝化速率的估计值）进行比较表明，该方法低估了真实硝化速率。对此进行了讨论。
