Pan P, Umbreit W W
J Bacteriol. 1972 Mar;109(3):1149-55. doi: 10.1128/jb.109.3.1149-1155.1972.
Nitrosomonas europaea, Nitrobacter agilis, Thiobacillus denitrificans, T. neapolitanus, and T. thioparus (all obligate autotrophic bacteria) have been grown in dialysis culture, on glucose salts media, in the absence of their specific inorganic energy source. Metabolic products for N. agilis grown on nitrite salts medium were identified as keto acids. Pyruvic acid inhibited this organism at 5 x 10(-5)m. Keto acids were not inhibitory for the thiobacilli grown on thiosulfate medium. However, when T. denitrificans was grown on glucose with dialysis, addition of 5 x 10(-4)m pyruvate inhibited growth. Thus, it appears pyruvate may be inhibitory for other autotrophs, as has been reported for T. thiooxidans.
欧洲亚硝化单胞菌、敏捷硝化杆菌、反硝化硫杆菌、那不勒斯硫杆菌和排硫硫杆菌（均为专性自养细菌）已在透析培养中，于葡萄糖盐培养基上，在缺乏其特定无机能源的情况下生长。在亚硝酸盐培养基上生长的敏捷硝化杆菌的代谢产物被鉴定为酮酸。丙酮酸在5×10⁻⁵m时抑制该生物体。酮酸对在硫代硫酸盐培养基上生长的硫杆菌无抑制作用。然而，当反硝化硫杆菌在透析条件下于葡萄糖上生长时，添加5×10⁻⁴m的丙酮酸会抑制生长。因此，正如对氧化硫硫杆菌所报道的那样，丙酮酸似乎可能对其他自养生物具有抑制作用。
