酚类微生物转化中的构效关系。
Structure-activity relationships in microbial transformation of phenols.
机构信息
Environmental Research Laboratory, U.S. Environmental Protection Agency, Athens, Georgia 30613.
出版信息
Appl Environ Microbiol. 1982 Jul;44(1):153-8. doi: 10.1128/aem.44.1.153-158.1982.
The second-order rate constants for the microbial transformation of a series of phenols were correlated with the physicochemical properties of the phenols. The compounds studied were phenol, p-methylphenol, p-chlorophenol, p-bromophenol, p-cyanophenol, p-nitrophenol, p-acetylphenol, and p-methoxyphenol. Phenol-grown cells of Pseudomonas putida U transformed these compounds. Microbial transformation rate constants ranged from (1.5 +/- 0.99) x 10 liter . organism . h for p-cyanophenol to (7.0 +/- 1.3) x 10 liter . organism . h for phenol. Linear regression analyses of rate constants and electronic, steric, and hydrophobic parameters showed that van der Waal's radii gave the best coefficient of determination (r = 0.956). Products identified by thin-layer chromatography and liquid chromatography indicated that the phenols were microbially oxidized to the corresponding catechols.
一系列酚类化合物的微生物转化的二级速率常数与酚类化合物的物理化学性质相关联。研究的化合物为苯酚、对甲基苯酚、对氯苯酚、对溴苯酚、对氰基苯酚、对硝基苯酚、对乙酰基苯酚和对甲氧基苯酚。恶臭假单胞菌（Pseudomonas putida U）的苯酚生长细胞转化了这些化合物。微生物转化速率常数的范围为：对氰基苯酚的（1.5 ± 0.99）×10 升 。 生物体 。 h 到苯酚的（7.0 ± 1.3）×10 升 。 生物体 。 h 。速率常数和电子、空间和疏水性参数的线性回归分析表明，范德华半径给出了最佳的决定系数（r = 0.956）。通过薄层层析和液相色谱鉴定的产物表明，这些酚类化合物被微生物氧化为相应的邻苯二酚。
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