Department of Biology, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9 and Division of Biology, Kansas State University, Manhattan, Kansas 66506.
Appl Environ Microbiol. 1982 Oct;44(4):965-71. doi: 10.1128/aem.44.4.965-971.1982.
Physiology and morphology of pole bean (Phaseolus vulgaris L. cv Kentucky Wonder) root nodules induced by two Rhizobium species of different cross-inoculation groups have been compared. Root nodules induced by Rhizobium sp. 127E15, which is a strain of the cowpea group Rhizobium, were pinkish, had irregular shapes, and were only partially effective. Their peak acetylene reduction activity was 4.36 mumol of C(2)H(4) formed per g of fresh nodules per h at 30 days after inoculation. The effective nodules induced by Rhizobium phaseoli 127K14, which is a strain of the bean group Rhizobium, were dark red, spherical, and showed peak acetylene reduction activity of 15.95 mumol of C(2)H(4) formed per g of fresh nodules per h at 15 days after inoculation. The partial effectiveness of 127E15-induced nodules was associated with fewer infected cells, a delay in the increase of bacteroid population within the host cells, abundance of cytoplasmic vesicles in the host cells, more bacteroids within a membrane envelope (peribacteroid membrane), and the inability of bacteroids to completely fill up the host cytoplasmic space. The 127K14-induced nodules were fully mature, with host cells filled with bacteroids by 12 days after inoculation. In contrast, the 127E15-induced nodules did not reach a similar developmental stage even 30 days after inoculation.
已比较了由两种不同交叉接种群的根瘤菌诱导的菜豆（Phaseolus vulgaris L. cv Kentucky Wonder）根瘤的生理学和形态学。由根瘤菌 127E15 诱导的根瘤呈粉红色，形状不规则，仅部分有效。它们的乙炔还原活性峰值为 30 天接种后每克新鲜根瘤每小时形成 4.36 微摩尔 C 2 H 4 。由根瘤菌 127K14 诱导的有效根瘤呈深紫红色，球形，接种后 15 天乙炔还原活性峰值为每克新鲜根瘤每小时形成 15.95 微摩尔 C 2 H 4 。127E15 诱导的根瘤部分有效与以下因素有关：感染细胞较少、宿主细胞内类菌体数量增加延迟、宿主细胞中细胞质小泡丰富、包膜内类菌体（类周膜）较多以及类菌体无法完全填满宿主细胞质空间。127K14 诱导的根瘤完全成熟，接种后 12 天宿主细胞充满类菌体。相比之下，即使在接种后 30 天，127E15 诱导的根瘤也未达到类似的发育阶段。
