Department of Marine Microbiology, University of Göteborg, S-413 19 Göteborg, Sweden.
Appl Environ Microbiol. 1982 Nov;44(5):1196-204. doi: 10.1128/aem.44.5.1196-1204.1982.
Microbial biofilm development was followed under growth conditions similar to those of a projected salinity power plant. Microscope glass cover slips were piled in biofilm reactors to imitate the membrane stacks in such a plant. A staining technique closely correlating absorbance values with biofilm dry weight was used for the study. Generally, the biofilms consisted of solitary and filamentous bacteria which were evenly distributed with considerable amounts of various protozoa and entrapped debris of organic origin. Protozoa predation was shown to decrease the amount of biofilm produced. The biofilm development lag phase was longer at lower temperatures. The subsequent growth phase was approximately arithmetic until stationary phase appeared. Adaptation of a hyperbolic saturation function gave curves that agreed well with the logarithm of the amount of biofilm as a function of time. Increased flow velocity, temperature, and nutrient concentration increased the biofilm production rate. An exponential relationship was shown between biofilm production rate and flow velocity within the range of 0 to 15 cm s. Intervals in which the biofilms were exposed to fresh water decreased the biofilm production rate more than four times. If the cover slips were inoculated with untreated seawater for 24 h, subsequent UV treatment had an insignificant effect on the biofilm formation.
在类似于预期的盐水发电厂的生长条件下，研究了微生物生物膜的发展。将显微镜玻璃盖玻片堆积在生物膜反应器中，以模拟该工厂中的膜堆。使用一种与生物膜干重密切相关的吸光度值的染色技术进行了研究。通常，生物膜由单独的和丝状细菌组成，这些细菌均匀分布，并且含有大量各种原生动物和有机来源的截留碎屑。原生动物捕食被证明会减少生物膜的产生量。较低的温度会延长生物膜的滞后阶段。随后的生长阶段大约是算术级，直到出现稳定阶段。双曲线饱和函数的适应给出了与生物膜量随时间的对数的曲线很好地吻合的曲线。流速、温度和营养浓度的增加会增加生物膜的产生速率。在 0 到 15 cm/s 的范围内，生物膜产生速率与流速之间呈指数关系。生物膜暴露于淡水的间隔时间会使生物膜的产生速率降低四倍以上。如果将盖玻片用未经处理的海水接种 24 小时，随后的 UV 处理对生物膜的形成没有明显影响。
