Ridgway H F, Rigby M G, Argo D G
Appl Environ Microbiol. 1984 Jan;47(1):61-7. doi: 10.1128/aem.47.1.61-67.1984.
The kinetics of adhesion of a Mycobacterium sp. to cellulose diacetate reverse-osmosis membranes is described. This Mycobacterium sp. (strain BT2-4) was previously implicated in the initial stages of reverse-osmosis membrane biofouling at a wastewater reclamation facility. Adhesion of BT2-4 cells to the cellulose diacetate membrane surfaces occurred within 1 to 2 h at 30 degrees C and exhibited saturation-type kinetics which conformed closely to the Langmuir adsorption isotherm (Pearson r correlation coefficient = 0.977), a mathematical expression describing the partitioning of substances between a solution and solid-liquid interface. This suggests that the cellulose diacetate membrane surfaces may possess a finite number of available binding sites to which the mycobacteria can adhere. Treatment of the attached mycobacteria with different enzymes suggested that cell surface polypeptides, alpha-1, 4- or alpha-1,6-linked glucan polymers, and carboxyl ester bond-containing substances (possibly peptidoglycolipids) may be involved in mycobacterial adhesion. The possible implication of these findings for reverse-osmosis membrane biofouling are discussed.
描述了一种分枝杆菌对二醋酸纤维素反渗透膜的粘附动力学。这种分枝杆菌（菌株BT2-4）先前被认为与一家废水回收设施中反渗透膜生物污染的初始阶段有关。在30℃下，BT2-4细胞在1至2小时内粘附到二醋酸纤维素膜表面，并表现出饱和型动力学，与朗缪尔吸附等温线密切相符（皮尔逊r相关系数=0.977），这是一个描述物质在溶液和固液界面之间分配的数学表达式。这表明二醋酸纤维素膜表面可能拥有有限数量的可供分枝杆菌粘附的结合位点。用不同酶处理附着的分枝杆菌表明，细胞表面多肽、α-1,4-或α-1,6-连接的葡聚糖聚合物以及含羧基酯键的物质（可能是肽糖脂）可能参与分枝杆菌的粘附。讨论了这些发现对反渗透膜生物污染的可能影响。
