丙酮丁醇梭菌的自溶活性和丁醇耐受性。
Autolytic Activity and Butanol Tolerance of Clostridium acetobutylicum.
机构信息
Department of Microbiology, University of Cape Town, Rondebosch, 7700 South Africa.
出版信息
Appl Environ Microbiol. 1982 Dec;44(6):1277-81. doi: 10.1128/aem.44.6.1277-1281.1982.
The effects of acetone and butanol on the growth of vegetative cells and the stability of swollen-phase bright-stationary-phase cells (clostridial forms) of Clostridium acetobutylicum P262 and an autolytic deficient mutant (lyt-1) were investigated. There was little difference in the sensitivity of strain P262 and the lyt-1 mutant vegetative cells and clostridial forms to acetone. The stability of the different morphological stages was unaffected by acetone concentrations far in excess of those encountered in factory fermentations. Butanol concentrations between 7 and 16 g/liter, which are within the range obtained in industrial fermentations, increased the degeneration of strain P262 clostridial forms but had no effect on the stability of lyt-1 clostridial forms which never underwent autolysis. Vegetative cells of the lyt-1 mutant were able to grow in higher concentrations of butanol than strain P262 vegetative cells. It was concluded that there is a relationship between butanol tolerance and autolytic activity.
研究了丙酮和丁醇对丙酮丁醇梭菌 P262 及其自溶缺陷突变体（lyt-1）的营养细胞生长和膨胀相稳定期细胞（梭菌形态）稳定性的影响。P262 菌株和 lyt-1 突变体营养细胞和梭菌形态对丙酮的敏感性差异很小。在远高于工厂发酵中遇到的浓度下，丙酮对不同形态阶段的稳定性没有影响。工业发酵中获得的 7 至 16 克/升范围内的丁醇浓度增加了 P262 梭菌形态的退化，但对 lyt-1 梭菌形态的稳定性没有影响，因为 lyt-1 梭菌形态从不发生自溶。lyt-1 突变体的营养细胞能够在比 P262 营养细胞更高浓度的丁醇中生长。结论是，丁醇耐受性和自溶活性之间存在关系。
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