Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
Appl Environ Microbiol. 1982 Jun;43(6):1434-9. doi: 10.1128/aem.43.6.1434-1439.1982.
The morphological and cytological changes which occurred in Clostridium acetobutylicum P262 during the production of acetone, butanol, and ethanol in an industrial fermentation medium were identified and correlated with the growth and physiological changes. The swollen, cigar-shaped clostridial forms were involved in the conversion of acids to neutral solvents, and there was a correlation between the number of clostridial forms and the production of solvents. Sporulation mutants which were unable to form clostridial stages (cls mutants) did not produce solvents. Oligosporogenous mutants which showed reduced clostridial stage formation produced intermediate levels of solvents. Sporulation mutants blocked after the clostridial stage, which were unable to form mature spores (spo mutants), produced normal levels of solvents.
在工业发酵培养基中生产丙酮、丁醇和乙醇过程中，鉴定了丙酮丁醇梭菌 P262 发生的形态和细胞学变化，并将其与生长和生理变化相关联。肿胀的雪茄形梭状芽孢杆菌形态参与了酸到中性溶剂的转化，梭状芽孢杆菌形态的数量与溶剂的生产之间存在相关性。无法形成梭状芽孢杆菌阶段的芽孢形成突变体（cls 突变体）不产生溶剂。形成梭状芽孢杆菌阶段减少的寡孢形成突变体产生中间水平的溶剂。在梭状芽孢杆菌阶段后被阻断、无法形成成熟孢子的芽孢形成突变体（spo 突变体）产生正常水平的溶剂。
