Biotechnology and Chemistry Groups, Eastern Laboratory, Forintek Canada Corp., Ottawa, Ontario, Canada K1G 3Z5.
Appl Environ Microbiol. 1983 Jan;45(1):153-60. doi: 10.1128/aem.45.1.153-160.1983.
Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluble fractions from steam-exploded aspen, solvent-extracted aspen, and commercial xylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly cellulolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, at a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae.
半纤维素衍生糖可从各种预处理木质素底物中获得，如蒸汽爆破杨木的水溶性级分、溶剂萃取杨木和商业木聚糖。这些级分通过商业酶制剂和八种高度纤维素分解真菌的培养滤液进行酶水解。通过高压液相色谱法测定释放的糖。在 10%底物浓度下，超过 30%的半纤维素级分可水解为单糖。这些半纤维素水解物被用作丙酮丁醇梭菌和肺炎克雷伯菌生长的基质。尽管用肺炎克雷伯菌可以将 1%底物浓度下的半纤维素级分中的 50%以上转化为 2,3-丁二醇、乙醇和乙酸，但用丙酮丁醇梭菌获得的丁醇值相对较低。
