Yu E K, Saddler J N
Appl Environ Microbiol. 1983 Sep;46(3):630-5. doi: 10.1128/aem.46.3.630-635.1983.
The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and 27 g of butanediol per liter, respectively. The efficiency of bioconversion decreased with increasing sugar substrate concentrations (up to 200 g/liter). Butanediol production at low sugar substrate concentrations was less efficient when the organism was grown under aerobic conditions; however, final butanediol values were higher for cultures grown on an initial sugar concentration of 150 g/liter, particularly when the inoculum was first acclimatized to high sugar levels. When a double fed-batch approach (daily additions of sugars together with yeast extract) was used under aerobic conditions, up to 88 and 113 g of combined butanediol and acetyl methyl carbinol per liter could be obtained from the utilization of 190 g of D-xylose and 226 g of D-glucose per liter, respectively.
研究了肺炎克雷伯菌在高糖浓度下将木聚糖半纤维素中的糖生物转化为2,3-丁二醇的过程。当肺炎克雷伯菌在有限空气条件下、添加乙酸的情况下生长时，每升50克的D-葡萄糖和D-木糖可分别转化为每升25克和27克的丁二醇。生物转化效率随着糖底物浓度的增加（高达200克/升）而降低。当该微生物在有氧条件下生长时，低糖底物浓度下的丁二醇产量效率较低；然而，初始糖浓度为150克/升的培养物最终丁二醇产量更高，特别是当接种物首先适应高糖水平时。当在有氧条件下采用双补料分批培养法（每天添加糖和酵母提取物）时，每升分别利用190克D-木糖和226克D-葡萄糖，可分别获得高达每升88克和113克的丁二醇和乙酰甲基甲醇的混合物。
