Department of Genetics, University of the Witwatersrand, Johannesburg 2001, South Africa, and Laboratoria voor Genetika en voor Histologie, Rijksuniversiteit Gent, B-9000 Gent, Belgium.
Appl Environ Microbiol. 1983 May;45(5):1526-32. doi: 10.1128/aem.45.5.1526-1532.1983.
Eighteen strains of Agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. Of six agrocin-producing strains, one (D286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type Ti plasmids. Sensitivity to agrocin D286 was found to map in the 11- to 18-megadalton region of the nopaline Ti plasmid pTiC58. The agrocin was partially purified, and its physical characteristics were consistent with its being a nucleotide, as is agrocin 84. Agrocin D286 was shown to inhibit DNA, RNA, and protein syntheses. Strain D286 spontaneously lost its pathogenicity, and its potential for use in the biological control of crown gall is discussed.
从冠瘿中分离出的 18 株根癌农杆菌被测试其产生农杆菌素的能力。在 6 株产生农杆菌素的菌株中，有 1 株（D286）产生了一种广谱农杆菌素，能有效对抗携带胭脂碱、章鱼碱和农杆菌素 Ti 质粒的菌株。对 agrocin D286 的敏感性被发现定位于胭脂碱 Ti 质粒 pTiC58 的 11-18 兆道尔顿区域。农杆菌素 D286 被部分纯化，其物理特性与其作为核苷酸一致，与 agrocin 84 相同。农杆菌素 D286 被证明能抑制 DNA、RNA 和蛋白质的合成。D286 菌株自发失去了其致病性，并讨论了其在生物防治冠瘿病中的潜在用途。
