Engler G, Holsters M, Van Montagu M, Schell J, Hernalsteens J P
Mol Gen Genet. 1975 Jul 10;138(4):345-9. doi: 10.1007/BF00264804.
It was shown for some oncogenic Agrobacterium tumefaciens strains that agrocin 84 sensitivity is determined by the presence of a large closed circular DNA plasmid, called the Ti-plasmid. Whereas wild-type strain C58 is agrocin 84 sensitive, all Ti-plasmid cured derivatives were found to be fully resistant. Moreover all independently isolated agrocin 84 resistant colonies were stably non-oncogenic and plasmid negative. In a growth experiment carried out at 37 degrees C it was shown that the kinetics of appearance of non-oncogenic cells on the one hand and of agrocin 84 resistant cells on the other were identical. The fact that not all oncogenic, plasmid harbouring, Agrobacterium tumefaciens strains are sensitive to agrocin 84, points to the possibility that the genes determining agrocin 84 sensitivity are not essential for tumor-inducing ability.
已表明，对于某些致癌性根癌农杆菌菌株，农杆菌素84敏感性由一种称为Ti质粒的大型闭合环状DNA质粒的存在所决定。野生型菌株C58对农杆菌素84敏感，而所有Ti质粒消除衍生物均被发现完全抗性。此外，所有独立分离的农杆菌素84抗性菌落均稳定地无致癌性且无质粒。在37℃进行的生长实验表明，一方面非致癌细胞的出现动力学与另一方面农杆菌素84抗性细胞的出现动力学相同。并非所有携带质粒的致癌性根癌农杆菌菌株都对农杆菌素84敏感，这一事实表明，决定农杆菌素84敏感性的基因对于肿瘤诱导能力并非必不可少。
