Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, Beaverton, Oregon 97006.
Appl Environ Microbiol. 1983 Jul;46(1):260-3. doi: 10.1128/aem.46.1.260-263.1983.
A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryons into their constituent homokaryotic strains could be effected through protoplast release and formation of colonies on regeneration agar.
两种商业酶制剂被用于从黄孢原毛平革菌 16 小时龄的菌丝体中释放原生质体。当原生质体在含有 10%山梨醇和 3%琼脂的培养基中平板培养时，可获得高达 5%的再生频率。聚乙二醇-Ca 中的原生质体融合不同营养缺陷型菌株产生异核体。通过原生质体释放和在再生琼脂上形成菌落，可以将异核体分离成其组成的同核菌株。
