聚乙二醇诱导的小链霉菌或抗生链霉菌原生质体高频融合
High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol.
作者信息
Ochi K, Hitchcock M J, Katz E
出版信息
J Bacteriol. 1979 Sep;139(3):984-92. doi: 10.1128/jb.139.3.984-992.1979.
Abstract
Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed.
摘要
建立了小链霉菌和抗生链霉菌原生质体再生为菌丝体形式的条件。再生是在含有蔗糖、氯化钙、氯化镁和低水平磷酸盐的高渗培养基中完成的。聚乙二醇4000（42%，重量/体积）诱导了来自小链霉菌或抗生链霉菌营养缺陷型菌株的原生质体的高频融合。融合程序导致的基因转移频率因所检测的营养缺陷型菌株而异。与小链霉菌营养缺陷型菌株融合导致形成真正的原养型重组体。对抗生链霉菌的类似研究表明，既形成了稳定的原养型重组体，也形成了异核体。
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