原生质体融合提高链霉菌属木质素降解能力。
Enhancement of Lignin Degradation in Streptomyces spp. by Protoplast Fusion.
机构信息
Department of Bacteriology and Biochemistry, University of Idaho, Moscow, Idaho 83843.
出版信息
Appl Environ Microbiol. 1984 Feb;47(2):439-40. doi: 10.1128/aem.47.2.439-440.1984.
Abstract
Protoplast fusion was investigated as a technique for genetically manipulating two lignin-degrading Streptomyces strains, Streptomyces viridosporus T7A and Streptomyces setonii 75Vi2. Four of 19 recombinants tested showed enhanced production of acid-precipitable polymeric lignin (APPL), producing 155 to 264% more APPL from corn stover lignocellulose than was produced by the wild-type S. viridosporus T7A. APPLs are lignin degradation intermediates known to be potentially valuable chemical products produced by bioconversion of lignin with Streptomyces spp. The prospects of utilizing protoplast fusion to construct APPL-overproducing Streptomyces strains was considered especially promising.
摘要
原生质体融合被研究作为一种遗传操作的技术，用于两种木质素降解链霉菌菌株，即变色链霉菌 T7A 和链霉菌 setonii 75Vi2。在测试的 19 个重组体中，有 4 个显示出增强的酸沉淀聚合木质素（APPL）的生产，从玉米秸秆木质纤维素中产生的 APPL 比野生型变色链霉菌 T7A 多 155%至 264%。APPLs 是木质素降解中间产物，已知是用链霉菌属进行木质素生物转化时可能有价值的化学产品。利用原生质体融合构建 APPL 过量产生的链霉菌菌株的前景被认为是特别有希望的。
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本文引用的文献
1
Lignin Degradation by Streptomyces viridosporus: Isolation and Characterization of a New Polymeric Lignin Degradation Intermediate.链霉菌属viridosporus 对木质素的降解：一种新型聚合木质素降解中间产物的分离与鉴定。
Appl Environ Microbiol. 1983 Mar;45(3):898-904. doi: 10.1128/aem.45.3.898-904.1983.
2
Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.链霉菌中的原生质体融合：高效基因重组和细胞再生的条件
J Gen Microbiol. 1981 Nov;127(1):137-46. doi: 10.1099/00221287-127-1-137.
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Genetic studies with bacterial protoplasts.细菌原生质体的遗传学研究。
Annu Rev Microbiol. 1981;35:237-72. doi: 10.1146/annurev.mi.35.100181.001321.
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Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.链霉菌原生质体的形成与再生：培养条件及形态学研究
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Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.通过原生质体融合和细胞再生实现弗氏链霉菌中的基因重组。
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Lignocellulose decomposition by selected streptomyces strains.特定链霉菌菌株对木质纤维素的分解
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