Department of Plant and Soil Biology, University of California, Berkeley, California 94720.
Appl Environ Microbiol. 1984 Oct;48(4):802-6. doi: 10.1128/aem.48.4.802-806.1984.
A potential for heterotrophic nitrification was identified in soil from a mature conifer forest and from a clear-cut site. Potential rates of NO(2) production were determined separately from those of NO(3) by using acetylene to block autotrophic NH(4) oxidation and chlorate to block NO(2) oxidation to NO(3) in soil slurries. Rates of NO(2) production were similar in soil from the forest and the clear-cut site and were strongly inhibited by acetylene. The rate of NO(3) production was much greater than that of NO(2) production, and NO(3) production was not significantly affected by acetylene or chlorate. Nitrate production was partially inhibited by cycloheximide, but was not significantly reduced by streptomycin. Neither the addition of ammonium nor the addition of peptone stimulated NO(3) production. N labeling of the NH(4) pool demonstrated that NO(3) was not coming from NH(4). The potential for heterotrophic nitrification in these forest soils was greater than that for autotrophic nitrification.
在成熟的针叶林土壤和皆伐迹地上的土壤中发现了异养硝化的潜力。通过使用乙炔来阻止自养 NH4氧化，以及使用氯酸盐来阻止 NO2氧化为 NO3，分别测定了 NO2和 NO3的产生速率。森林和皆伐迹地上的土壤中 NO2的产生速率相似，并且乙炔对其有强烈的抑制作用。NO3的产生速率远大于 NO2的产生速率，并且乙炔或氯酸盐对其没有显著影响。环丝氨酸部分抑制了硝酸盐的产生，但链霉素没有显著降低其产生。添加铵或蛋白胨均没有刺激 NO3的产生。NH4库的 N 标记表明 NO3不是来自 NH4。这些森林土壤中的异养硝化潜力大于自养硝化潜力。
