Department of Microbiology and Immunology and Electron Microscope Facility, College of Agriculture, University of Arizona, Tucson, Arizona 85721.
Appl Environ Microbiol. 1985 Feb;49(2):446-50. doi: 10.1128/aem.49.2.446-450.1985.
Fixation of cellulose-acetate membranes with either glutaraldehyde-osmium tetroxide or glutaraldehyde-ruthenium tetroxide resulted in extensive electron beam damage. Beam damage was eliminated and the bacterial surface structure was preserved, however, when cellulose-acetate membranes were fixed with glutaraldehyderuthenium tetroxide and treated successively with thiocarbohydrazide and osmium tetroxide.
用戊二醛-锇四氧化物或戊二醛-钌四氧化物固定醋酸纤维素膜会导致严重的电子束损伤。然而,当醋酸纤维素膜用戊二醛-钌四氧化物固定并用硫代卡巴肼和锇四氧化物连续处理时,电子束损伤被消除,并且细菌表面结构得到保存。