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改进醋酸纤维素反渗透膜用于扫描电子显微镜的固定。

Improved fixation of cellulose-acetate reverse-osmosis membrane for scanning electron microscopy.

机构信息

Department of Microbiology and Immunology and Electron Microscope Facility, College of Agriculture, University of Arizona, Tucson, Arizona 85721.

出版信息

Appl Environ Microbiol. 1985 Feb;49(2):446-50. doi: 10.1128/aem.49.2.446-450.1985.

DOI:10.1128/aem.49.2.446-450.1985
PMID:16346735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238424/
Abstract

Fixation of cellulose-acetate membranes with either glutaraldehyde-osmium tetroxide or glutaraldehyde-ruthenium tetroxide resulted in extensive electron beam damage. Beam damage was eliminated and the bacterial surface structure was preserved, however, when cellulose-acetate membranes were fixed with glutaraldehyderuthenium tetroxide and treated successively with thiocarbohydrazide and osmium tetroxide.

摘要

用戊二醛-锇四氧化物或戊二醛-钌四氧化物固定醋酸纤维素膜会导致严重的电子束损伤。然而,当醋酸纤维素膜用戊二醛-钌四氧化物固定并用硫代卡巴肼和锇四氧化物连续处理时,电子束损伤被消除,并且细菌表面结构得到保存。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/56b6871778f9/aem00148-0199-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/30cff7455d39/aem00148-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/cab7f6347df8/aem00148-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/79f266b48398/aem00148-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/4c572f37441d/aem00148-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/9438e5aec7df/aem00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/56b6871778f9/aem00148-0199-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/30cff7455d39/aem00148-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/cab7f6347df8/aem00148-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/79f266b48398/aem00148-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/4c572f37441d/aem00148-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/9438e5aec7df/aem00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/007f/238424/56b6871778f9/aem00148-0199-b.jpg

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本文引用的文献

1
Microbial fouling of reverse-osmosis membranes used in advanced wastewater treatment technology: chemical, bacteriological, and ultrastructural analyses.先进废水处理技术中使用的反渗透膜的微生物污染:化学、细菌学和超微结构分析。
Appl Environ Microbiol. 1983 Mar;45(3):1066-84. doi: 10.1128/aem.45.3.1066-1084.1983.