Corvellec M R, Lalague E D, Pelletier L, Haviernick S, Derghazarian C, Cousineau G H
J Microsc. 1983 Nov;132(Pt 2):229-33. doi: 10.1111/j.1365-2818.1983.tb04277.x.
Normal human leucocytes, successively treated with glutaraldehyde-tannic acid-osmium tetroxide-thiocarbohydrazide-osmium tetroxide-thiocarbohydrazide-osmium tetroxide, were prepared for scanning electron microscopy observation. These cells produced well-contrasted, non-charging scanning images compatible with metal-evaporated material. Further, the mononuclear and polymorphonuclear cells resisted shrinkage during dehydration and critical point drying, thus allowing much improved images at high magnification than those covered with evaporated metal. In all cases at least a second thiocarbohydrazide-osmium tetroxide treatment could not be avoided.
将正常人白细胞依次用戊二醛 - 单宁酸 - 四氧化锇 - 硫代碳酰肼 - 四氧化锇 - 硫代碳酰肼 - 四氧化锇处理后，制备用于扫描电子显微镜观察的样本。这些细胞产生了对比度良好、无电荷的扫描图像，与金属蒸发材料兼容。此外，单核细胞和多形核细胞在脱水和临界点干燥过程中抵抗收缩，因此在高倍放大下能获得比覆盖蒸发金属的细胞更好的图像。在所有情况下，至少第二次硫代碳酰肼 - 四氧化锇处理是不可避免的。
