阿魏酸和苯甲酸的甲烷发酵：厌氧降解途径。
Methane fermentation of ferulate and benzoate: anaerobic degradation pathways.
机构信息
Department of Civil Engineering, Stanford University, Stanford, California 94305, and Department of Environmental Medicine and Department of Microbiology, New York University Medical Center, New York, New York 10016.
出版信息
Appl Environ Microbiol. 1985 Aug;50(2):292-7. doi: 10.1128/aem.50.2.292-297.1985.
The anaerobic biodegradation of ferulate and benzoate in stabilized methanogenic consortia was examined in detail. Up to 99% of the ferulate and 98% of the benzoate were converted to carbon dioxide and methane. Methanogenesis was inhibited with 2-bromoethanesulfonic acid, which reduced the gas production and enhanced the buildup of intermediates. Use of high-performance liquid chromatography and two gas chromatographic procedures yielded identification of the following compounds: caffeate, p-hydroxycinnamate, cinnamate, phenylpropionate, phenylacetate, benzoate, and toluene during ferulate degradation; and benzene, cyclohexane, methylcyclohexane, cyclohexanecarboxylate, cyclohexanone, 1-methylcyclohexanone, pimelate, adipate, succinate, lactate, heptanoate, caproate, isocaproate, valerate, butyrate, isobutyrate, propionate, and acetate during the degradation of either benzoate or ferulate. Based on the identification of the above compounds, more complete reductive pathways for ferulate and benzoate are proposed.
详细研究了稳定产甲烷菌群中阿魏酸和苯甲酸的厌氧生物降解。阿魏酸和苯甲酸分别有 99%和 98%转化为二氧化碳和甲烷。使用 2-溴乙磺酸抑制产甲烷作用，降低了气体产量并促进了中间产物的积累。使用高效液相色谱和两种气相色谱程序鉴定了以下化合物：在阿魏酸降解过程中产生咖啡酸、对羟基肉桂酸、肉桂酸、苯丙酸、苯乙酸、苯甲酸和甲苯；在苯甲酸或阿魏酸降解过程中产生苯、环己烷、甲基环己烷、环己烷羧酸、环己酮、1-甲基环己酮、延胡索酸、己二酸、琥珀酸、乳酸、庚酸、己酸、异丁酸、戊酸、丁酸、异丁酸、丙酸和乙酸。基于上述化合物的鉴定，提出了更完整的阿魏酸和苯甲酸的还原途径。
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