Environmental Engineering and Science, Department of Civil Engineering, Stanford University, Stanford, California 94305.
Appl Environ Microbiol. 1986 Jul;52(1):200-2. doi: 10.1128/aem.52.1.200-202.1986.
Toluene and benzene were anaerobically transformed and eventually mineralized in mixed methanogenic cultures. However, the source of oxygen for the initial oxidation step had been unknown, owing to the presence of both methanol and water. No exogenous electron acceptors other than carbon dioxide, toluene, and benzene were present in the defined mineral medium. Through the use of O-labeled water, the oxygen incorporated into the monoaromatic compounds was shown to come from water. The cresol from the toluene and the phenol from the benzene contained up to 8% O label after incubation in 9% O-labeled medium. Gas chromatography-mass spectrometry was used to detect the O-labeled aromatic metabolites.
甲苯和苯在混合产甲烷菌培养物中被厌氧转化，最终被矿化。然而，由于甲醇和水的存在，最初氧化步骤的氧源一直未知。在所定义的矿物培养基中，除了二氧化碳、甲苯和苯之外，没有其他外源电子受体。通过使用 O 标记水，表明进入单芳烃化合物的氧来自水。在 9%O 标记培养基中孵育后，甲苯中的间甲酚和苯中的苯酚中含有高达 8%的 O 标记。使用气相色谱-质谱联用仪检测到 O 标记的芳香族代谢物。
