Department of Environmental Sciences, University of Warwick, Coventry CV4 7AL, West Midlands, United Kingdom.
Appl Environ Microbiol. 1986 Jan;51(1):44-51. doi: 10.1128/aem.51.1.44-51.1986.
Four Pseudomonas species and two Alcaligenes species were isolated from soil with a capacity to grow on halogenated alkanoic acids. They were shown to contain one of five large plasmids. The plasmids had molecular weights ranging from 98,800 to 190,000. They were associated with the ability to utilize the halogenated substrates 2-monochloropropionic acid and 2-monochloroacetic acid and with resistance towards one or more of the heavy metals mercury, selenium, and tellurium. The largest plasmid, pUU204, was shown to be unstable in continuous-flow culture when the organism was supplied with succinate as the sole carbon source. The dehalogenase gene associated with pUU204 appeared to be readily transferred to an incP group plasmid, R68-45.
从能够以卤代烷酸为碳源生长的土壤中分离到四种假单胞菌和两种产碱杆菌。它们都含有 5 种大质粒中的一种。这些质粒的分子量范围从 98800 到 190000。它们与利用卤代底物 2-氯丙酸和 2-氯乙酸的能力以及对汞、硒和碲等一种或多种重金属的抗性有关。最大的质粒 pUU204 在以琥珀酸为唯一碳源的连续流动培养中表现出不稳定性。与 pUU204 相关的脱卤酶基因似乎很容易转移到 incP 组质粒 R68-45 上。
