Wheatcroft R, Williams P A
J Gen Microbiol. 1981 Jun;124(2):433-7. doi: 10.1099/00221287-124-2-433.
Rapid methods for the analysis of degradative plasmids in Pseudomonas are described. Bacterial lysates prepared with alkaline sodium dodecyl sulphate were vigorously stirred in the presence of antifoam agent. Samples were subjected to agarose gel electrophoresis to detect plasmid DNA. After a short period of centrifugation on a sucrose gradient, the lysates yielded plasmid DNA of sufficient purity for restriction endonuclease digestion without further treatment. The methods have proved most useful in the extraction of previously undetected plasmid DNA, some of which appears to be unstable.
本文描述了分析假单胞菌中降解性质粒的快速方法。用碱性十二烷基硫酸钠制备的细菌裂解物在消泡剂存在下剧烈搅拌。样品进行琼脂糖凝胶电泳以检测质粒DNA。在蔗糖梯度上短暂离心后，裂解物产生的质粒DNA纯度足以用于限制性内切酶消化，无需进一步处理。这些方法已被证明在提取先前未检测到的质粒DNA方面非常有用，其中一些质粒DNA似乎不稳定。
