Department of Chemical Engineering, McGill University, Montreal, Quebec H3A 2A7, Canada.
Appl Environ Microbiol. 1986 Jul;52(1):152-6. doi: 10.1128/aem.52.1.152-156.1986.
Nonvolatile residue (NVR), a waste stream from the manufacture of nylon 6'6', contains mainly small carboxylic acids and alcohols, making it a potential fermentation substrate. Above a concentration of 1.3% (wt/vol), NVR inhibited the growth of all microorganisms tested. The most inhibitory of the major NVR components were the monocarboxylic acids (C(4) to C(6)) and epsilon-caprolactone. The inhibitory effects of NVR could be avoided by using a carbon-limited chemostat. Microorganisms were found that could use all of the major NVR components as carbon and energy sources. One such organism, Pseudomonas cepacia, was grown in a carbon-limited chemostat with a medium feed concentration of 20.5 g of NVR liter. At a dilution rate of 0.14 h the yield of biomass (Y(x/s), where x is biomass produced and s is substrate used) from NVR was 18% (neglecting the water content of NVR). It was concluded that NVR would be a suitable carbon source for certain industrial fermentation processes such as the production of poly-beta-hydroxybutyric acid.
不可挥发残渣(NVR)是尼龙 66 生产过程中的一种废水,主要含有小分子羧酸和醇,因此它是一种潜在的发酵基质。当浓度超过 1.3%(wt/vol)时,NVR 会抑制所有测试微生物的生长。NVR 中主要成分中抑制性最强的是单羧酸(C4 到 C6)和己内酯。采用碳限制恒化器可以避免 NVR 的抑制作用。研究发现,有些微生物可以将 NVR 的主要成分全部用作碳源和能源。其中一种微生物是假单胞菌(Pseudomonas cepacia),它在碳限制恒化器中以培养基进料浓度为 20.5 g/L 的 NVR 进行培养。在稀释率为 0.14 h 的条件下,从 NVR 中获得的生物质产率(Y(x/s),其中 x 是生物质产量,s 是基质用量)为 18%(忽略 NVR 中的水分含量)。因此,可以得出结论,NVR 是某些工业发酵过程(如聚-β-羟基丁酸的生产)的合适碳源。