优化泥炭水解物培养基以生产普鲁兰多糖。
Peat hydrolysate medium optimization for pullulan production.
机构信息
Department of Chemical Engineering, Laval University, Sainte-Foy, Quebec, Canada G1K 7P4.
出版信息
Appl Environ Microbiol. 1984 Jul;48(1):26-30. doi: 10.1128/aem.48.1.26-30.1984.
Peat hydrolysate, a diluted acid-autoclaved extract of peat, was used as a substrate for the production of the extracellular polysaccharide pullulan by three strains of Aureobasidium pullulans, 140B, 142, and 2552. It was found that the addition of (NH(4))(2)SO(4) and K(2)HPO(4) as sources of nitrogen and phosphate, respectively, is not necessary for the polysaccharide production. The economically optimized culture medium for large-scale production of pullulan contains peat hydrolysate, 0.05% NaCl, 0.02% MgSO(4), and 0.01% antifoam FG-10. The initial pH of peat hydrolysate medium is adjusted to its optimum value of 6.0 with Ca(OH)(2). The total ingredient cost for the production of each kilogram of pullulan with optimized medium is only 1/10 of that with the nonoptimized medium. In this study, a zero cost for peat hydrolysate was assumed, since it is an effluent of the peat and peat processing industries.
泥炭水解物是泥炭经酸高压蒸煮提取的稀酸浸出物，曾被用作三种出芽短梗霉（140B、142 和 2552）生产胞外多糖普鲁兰的基质。结果发现，添加（NH4）2SO4 和 K2HPO4 （分别作为氮源和磷源）对于多糖生产并非必需。用于大规模生产普鲁兰的经济优化培养基包含泥炭水解物、0.05%NaCl、0.02%MgSO4 和 0.01%消泡剂 FG-10。用 Ca（OH）2 将泥炭水解物培养基的初始 pH 值调至 6.0 的最佳值。用优化培养基生产每公斤普鲁兰的总成分成本仅是用非优化培养基的 1/10。在本研究中，假设泥炭水解物是零成本的，因为它是泥炭和泥炭加工行业的废水。
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