Departamento de Bioquímica, Facultades de Ciencias, Burjassot, Universitat de València, València, Spain.
Appl Environ Microbiol. 1986 Jul;52(1):157-60. doi: 10.1128/aem.52.1.157-160.1986.
The phytopathogenic bacterium Pseudomonas syringae produces a fluorescent pigment when it is grown in iron-deficient media. This pigment forms a very stable Fe(III) complex that was purified in this form by using a novel procedure based on ultrafiltration and column chromatography. The Fe(III) complex has a molecular weight of 1,100 and contains 1 mol of Fe(III). The pigment is composed of an amino acid moiety with three threonines, three serines, one lysine, delta-N-hydroxyornithine, and a quinoline-type fluorescent chromophore. These features and its stability constant (in the range of 10) suggest that the fluorescent pigment of P. syringae is related to the siderophores produced by another Pseudomonas species.
当植物病原细菌丁香假单胞菌在缺铁的培养基中生长时，会产生一种荧光色素。这种色素形成了一种非常稳定的 Fe(III) 配合物，可以通过一种新颖的基于超滤和柱层析的方法以这种形式进行纯化。该 Fe(III) 配合物的分子量为 1100，含有 1 摩尔的 Fe(III)。色素由一个具有三个苏氨酸、三个丝氨酸、一个赖氨酸、δ-N-羟基鸟氨酸和一个喹啉型荧光生色团的氨基酸部分组成。这些特征及其稳定常数（在 10 的范围内）表明，丁香假单胞菌的荧光色素与另一种假单胞菌产生的铁载体有关。
