高效液相色谱法比较细菌脂多糖。
Comparison of bacterial lipopolysaccharides by high-performance liquid chromatography.
机构信息
Department of Microbiology, University of Edinburgh, Edinburgh EH9 3JG, Scotland.
出版信息
Appl Environ Microbiol. 1986 Oct;52(4):948-50. doi: 10.1128/aem.52.4.948-950.1986.
A comparison of lipid-free polysaccharides from gram-negative bacteria was rapidly accomplished by using high-performance liquid chromatography of underivatized hydrolysates. Examination of a number of such products revealed that, contrary to earlier reports, Xanthomonas campestris lipopolysaccharide contained heptose, together with rhamnose and galactose, but not mannose. The polymers from the methanotrophs "Methylomonas albus" and "Methylosinus trichosporium" contained heptose and glucose, and that from a "Klebsiella aerogenes" strain contained heptose, glucose, and galactose. The absence of heptose from the lipopolysaccharide of Myxococcus xanthus was confirmed.
采用未衍生水解产物的高效液相色谱法，可快速比较革兰氏阴性细菌的无脂多糖。对许多此类产品的检查表明，与早期的报告相反，野油菜黄单胞菌脂多糖含有庚糖，以及鼠李糖和半乳糖，但不含甘露糖。产甲烷菌“白甲基单胞菌”和“三孢布拉氏霉菌”的聚合物含有庚糖和葡萄糖，而“产气克雷伯氏菌”菌株的聚合物含有庚糖、葡萄糖和半乳糖。黄粘球菌脂多糖中不含庚糖也得到了证实。
Zotero 插件