Volk W A
J Bacteriol. 1968 Mar;95(3):980-2. doi: 10.1128/jb.95.3.980-982.1968.
The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all preparations contained uronic acid, phosphate, and mannose in molar ratios of approximately 1:2:1, and glucose and rhamnose in more variable concentrations. Most lipopolysaccharides contained either xylose or fucose, but those extracted from X. sinensis and X. campestris contained neither xylose nor fucose.
用热的45%苯酚从属于黄单胞菌属的17个种的细胞壁脂多糖中提取脂多糖。纯化后，通过脂多糖的酸水解获得多糖的组分并进行定量分析。所获得的数据表明，所有制剂均含有糖醛酸、磷酸盐和甘露糖，其摩尔比约为1:2:1，葡萄糖和鼠李糖的浓度变化较大。大多数脂多糖含有木糖或岩藻糖，但从中华黄单胞菌和野油菜黄单胞菌中提取的脂多糖既不含木糖也不含岩藻糖。
