Empresa Brasileira de Pesquisa Agropecuaria, Centro Nacional de Pesquisa de Arroz e Feijão, Caixa Postal 179-74000 Goiânia, Goiás, Brazil; Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824; and Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.
Appl Environ Microbiol. 1986 Oct;52(4):954-6. doi: 10.1128/aem.52.4.954-956.1986.
The semienclosed tube culture technique of Gibson was modified to permit growth of common bean (Phaseolus vulgaris L.) roots in humid air, enabling enumeration of the homologous (nodule forming) symbiont, Rhizobium phaseoli, by the most-probable-number plant infection method. A bean genotype with improved nodulation characteristics was used as the plant host. This method of enumeration was accurate when tubes were scored 3 weeks after inoculation with several R. phaseoli strains diluted from aqueous suspensions, peat-based inoculants, or soil. A comparison of population sizes obtained by most-probable-number tube cultures and plate counts indicated that 1 to 3 viable cells of R. phaseoli were a sufficient inoculant to induce nodule formation.
吉本氏半封闭式管培养技术被改良，使普通菜豆（Phaseolus vulgaris L.）的根在高湿度空气中生长，从而可以通过最可能数植物感染法对同源（结瘤形成）共生体 Rhizobium phaseoli 进行计数。使用具有改良结瘤特性的菜豆基因型作为植物宿主。用几种从水悬浮液、泥炭基接种剂或土壤中稀释的 R. phaseoli 菌株接种后 3 周，对管进行评分时，这种计数方法非常准确。用最可能数管培养和平板计数获得的种群大小比较表明，1 到 3 个活的 R. phaseoli 细胞足以诱导结瘤。
