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Accuracy of a Plant-Infection Technique for Counting Populations of Rhizobium trifolii.一种用于计数三叶草根瘤菌种群的植物感染技术的准确性。
Appl Microbiol. 1963 Sep;11(5):377-83. doi: 10.1128/am.11.5.377-383.1963.
2
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Serologic characteristics of certain root-nodule bacteria of legumes.
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本文引用的文献

1
Application of Statistics to Problems in Bacteriology: IV. Experimental Comparison of the Dilution Method, the Plate Count, and the Direct Count for the Determination of Bacterial Populations.统计学在细菌学问题中的应用：IV. 用于测定细菌群体的稀释法、平板计数法和直接计数法的实验比较
J Bacteriol. 1935 Jun;29(6):609-34. doi: 10.1128/jb.29.6.609-634.1935.
2
The dilution assay of viruses.病毒的稀释试验
J Hyg (Lond). 1954 Jun;52(2):189-93. doi: 10.1017/s002217240002739x.

一种用于计数三叶草根瘤菌种群的植物感染技术的准确性。

Accuracy of a Plant-Infection Technique for Counting Populations of Rhizobium trifolii.

作者信息

Brockwell J

机构信息

Division of Plant Industry, CSIRO, Canberra, Australia.

出版信息

Appl Microbiol. 1963 Sep;11(5):377-83. doi: 10.1128/am.11.5.377-383.1963.

DOI:10.1128/am.11.5.377-383.1963
PMID:16349637
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1058010/
Abstract

A "plant-infection" technique for identification and estimation of populations of Rhizobium is described and compared with petri dish colony counts of the same bacterial populations. Provided that bacterial suspensions are agitated thoroughly, dilutions made at 4 C, and test plants grown on agar, the plant-infection technique is an accurate method of estimating R. trifolii in pure culture or when added to soil. The results are discussed in relation to previous investigations of the subject. Tables are presented which, when applied to the distribution of positive (nodulated) test plants in either fivefold or tenfold dilution series, permit calculation of most probable numbers, and confidence limits are stated.

摘要

本文描述了一种用于鉴定和估计根瘤菌种群的“植物感染”技术，并将其与相同细菌种群在培养皿上的菌落计数进行了比较。只要细菌悬液充分振荡、在4℃下进行稀释，且测试植物在琼脂上生长，那么植物感染技术就是一种准确估计纯培养物中或添加到土壤中的三叶草根瘤菌的方法。结合之前对该主题的研究对结果进行了讨论。文中给出了一些表格，当应用于五倍或十倍稀释系列中阳性（结瘤）测试植物的分布时，可用于计算最可能数，并给出了置信限。