Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1986 Nov;52(5):1026-30. doi: 10.1128/aem.52.5.1026-1030.1986.
Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially purified xylanase exceeded 2,000 IU/mg. Xylanase from typically pigmented strains appeared similar to that from color variants with respect to molecular weight, pH and temperature optima, and specific activity of purified (but not crude) enzyme. However, xylanase from typical strains made up only about 1.0% of total extracellular protein. Xylanase from strains of Cryptococcus albidus was associated with abundant proteins of about 43 kilodaltons and showed much lower specific activity.
从天然存在的出芽短梗霉颜色变体中提取的木聚糖酶与 20 到 21 千道尔顿的细胞外单体蛋白有关。木聚糖酶占总细胞外蛋白的近一半，产量高达每升 0.3 克木聚糖酶。部分纯化的木聚糖酶的比活超过 2000IU/mg。典型着色菌株的木聚糖酶与颜色变体的木聚糖酶在分子量、pH 和温度最佳值以及纯化（但非粗提）酶的比活方面相似。然而，典型菌株的木聚糖酶仅占总细胞外蛋白的约 1.0%。从白色隐球菌菌株中提取的木聚糖酶与大量约 43 千道尔顿的蛋白有关，且比活较低。
