Laboratorio de Bioquimica, Departamento de Biologia Celular, Pontificia Universidad Catolica de Chile, Casilla 114-D, Santiago, Chile.
Appl Environ Microbiol. 1986 Dec;52(6):1428-32. doi: 10.1128/aem.52.6.1428-1432.1986.
Bacterial strains were isolated on the basis of their ability to proliferate in a minimal medium containing one of a series of lignin-related compounds as the sole carbon and energy source. These included the aromatic monomers guaiacol, vanillic and coumaric acids, a dimer and a trimer possessing the arylglycerol-beta-aryl ether linkage, anisoin, and both the ether-soluble and -insoluble fractions of kraft lignin. The growth of the strains on each of these compounds was measured. The results showed that the metabolic properties of the strains varied according to the structure of the carbon sources used for their selection. Spectrophotometric tracings of the culture medium during the log phase of growth of one of the strains on the beta-O-4 dimer revealed decomposition with the release of guaiacol.
细菌菌株是根据其在含有一系列木质素相关化合物作为唯一碳源和能源的最小培养基中增殖的能力来分离的。这些化合物包括芳香单体愈创木酚、香草酸和香豆酸、具有芳基甘油-β-芳基醚键的二聚体和三聚体、阿魏酸以及 kraft 木质素的醚溶性和不溶性部分。测量了菌株在每种化合物上的生长情况。结果表明,菌株的代谢特性根据用于选择它们的碳源的结构而有所不同。在一株菌以β-O-4 二聚体生长的对数期,对培养液进行的分光光度追踪显示,该二聚体发生了分解,并释放出愈创木酚。