Department of Plant Genetics and Department of Isotopes, The Weizmann Institute of Science, Rehovot, Israel.
Appl Environ Microbiol. 1987 Feb;53(2):358-64. doi: 10.1128/aem.53.2.358-364.1987.
The enzyme-linked immunosorbent assay is suggested as a reliable, sensitive, and highly specific method for the identification and enumeration of Azospirillum brasilense Cd. As few as 10 CFU/ml can be practically identified by this method. At higher bacterial numbers, sensitivity increased linearly up to 5 x 10 CFU/ml, yielding useful standard curves. No cross-reaction was found either with different closely related Azospirillum strains or with other rhizosphere bacteria. The method allows for a specific identification of A. brasilense Cd. both in pure cultures and in mixtures with other bacterial species, even when the colony morphology is variable. The method was successfully applied to assess the degree of root colonization on various cereals by A. brasilense Cd.
酶联免疫吸附测定法被认为是一种可靠、敏感和高度特异的方法，可用于鉴定和计数巴西固氮螺菌 Cd。通过这种方法，实际上可以检测到低至 10 CFU/ml 的数量。在更高的细菌数量下，灵敏度呈线性增加，最高可达 5 x 10 CFU/ml，产生有用的标准曲线。该方法与不同的密切相关的巴西固氮螺菌菌株或其他根际细菌之间没有发现交叉反应。该方法允许对纯培养物和与其他细菌种类混合的巴西固氮螺菌 Cd 进行特异性鉴定，即使菌落形态可变。该方法成功地应用于评估巴西固氮螺菌 Cd 对各种谷物的根定植程度。
