Kishinevsky B, Bar-Joseph M
Can J Microbiol. 1978 Dec;24(12):1537-43. doi: 10.1139/m78-245.
The technique of enzyme-linked immunosorbent assay (ELISA) was used for serological identification of peanut Rhizobium strains both in cell suspension of pure culture and in single root nodules of groundnut (Arachis hypogaea) plants. Antisera of three peanut Rhizobium strains were tested against eight different Rhizobium isolates. Three serogroups identified by agglutination and immunodiffusion tests were confirmed by ELISA. In this experiment ELISA was more sensitive by four to six orders of magnitude than the agglutination and immunodiffusion tests and enabled the detection of Rhizobium antigens in cell suspensions of 10(4)-10(5) cells per millilitre. The reactions of culture and nodule antigens were identical for all strains investigated. ELISA enabled the precise typing of rhizobial isolates in single small root nodules. The minimum fresh weight of nodule tissue necessary to perform the ELISA test was 0.4 mg crushed in 1 ml of phosphate-buffered saline (PBS). ELISA was also successfully used for strain identification in mixed inoculated plants. One of the strains in each pair formed most of the nodules examined.
酶联免疫吸附测定（ELISA）技术用于在纯培养物的细胞悬液以及花生（落花生）植株的单个根瘤中对花生根瘤菌菌株进行血清学鉴定。三种花生根瘤菌菌株的抗血清针对八种不同的根瘤菌分离物进行了检测。通过凝集试验和免疫扩散试验鉴定的三个血清群通过ELISA得到了证实。在本实验中，ELISA的灵敏度比凝集试验和免疫扩散试验高4至6个数量级，能够检测出每毫升含有10⁴ - 10⁵个细胞的细胞悬液中的根瘤菌抗原。对于所有研究的菌株，培养物抗原和根瘤抗原的反应是相同的。ELISA能够对单个小根瘤中的根瘤菌分离物进行精确分型。进行ELISA检测所需的根瘤组织最小鲜重为0.4毫克，将其在1毫升磷酸盐缓冲盐水（PBS）中研磨。ELISA还成功用于混合接种植物中的菌株鉴定。每对菌株中的一种形成了所检查的大多数根瘤。
