杆状病毒多角体表面电荷的测量。
Measurement of surface charge of baculovirus polyhedra.
机构信息
Natural Environment Research Council, Institute of Virology, Oxford OX1 3SR, England.
出版信息
Appl Environ Microbiol. 1987 Mar;53(3):598-602. doi: 10.1128/aem.53.3.598-602.1987.
Abstract
The isoelectric points of three baculoviruses, Trichoplusia ni nuclear polyhedrosis virus (NPV), T. ni granulosis virus, and Spodoptera littoralis NPV were identified by cell electrophoresis. At neutral pH polyhedra were negatively charged. T. ni NPV polyhedra were reacted with a number of reagents which could potentially attach to or degrade their surface structure. This gave information on the components that contribute to the charge profile of T. ni NPV. This is discussed in relation to the use of polyhedra as biological control agents against insect pests.
摘要
三种杆状病毒，即夜蛾核多角体病毒（NPV）、夜蛾颗粒体病毒和小地老虎 NPV 的等电点通过细胞电泳法确定。在中性 pH 值下，多角体带负电荷。用一些可能附着在其表面结构上或降解其表面结构的试剂来处理夜蛾 NPV 多角体。这为确定夜蛾 NPV 多角体电荷分布特征的组成部分提供了信息。本文还讨论了将多角体作为防治鳞翅目害虫的生物防治剂的应用。
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