Microbiology Program, The Pennsylvania State University, University Park, Pennsylvania 16802.
Appl Environ Microbiol. 1987 Apr;53(4):887-8. doi: 10.1128/aem.53.4.887-888.1987.
A technique is described for obtaining most probable number estimates of the number of cells of a bacterial strain(s) when it is present in low numbers in the soil. The technique is based on the bacteriophage response that is elicited when a known number of bacteriophage for the bacterium of interest is incubated with soil dilutions in a nutrient broth. The technique was evaluated for use with gram-negative bacteria.
介绍了一种技术,用于在土壤中细菌数量较低时获得细菌菌株的最可能数估计值。该技术基于噬菌体反应,当用已知数量的感兴趣细菌的噬菌体与营养肉汤中的土壤稀释物孵育时,会引起噬菌体反应。该技术已评估用于革兰氏阴性菌。