Agricultural Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Monticello, Florida 32344; Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 ; and Department of Biological Sciences, Florida State University, Tallahassee, Florida 32306.
Appl Environ Microbiol. 1988 Jun;54(6):1570-4. doi: 10.1128/aem.54.6.1570-1574.1988.
The membrane phospholipid fatty acids (PLFAs) from several cocultures and a pure culture of Syntrophomonas wolfei were determined by capillary column gas chromatography. Cocultures of S. wolfei with a Desulfovibrio sp. contained PLFAs from both organisms, whereas PLFAs from a coculture with Methanospirillum hungatei contained very little biomass to analyze. The pure culture of S. wolfei grown on crotonate provided the best material for analysis of the PLFAs. The predominant PLFAs of S. wolfei were the monounsaturated 16:1omega7c and 16:1omega9c and the saturated 16:0 and 14:0. A low concentration of the diunsaturated 18:2omega6 was detected. The PLFA analysis provides additional information for consideration in the determination of the profile of PLFAs obtained from anaerobic environments. In addition, this information may aid in the understanding of the physiology and phylogeny of S. wolfei and other syntrophic bacteria.
采用毛细管柱气相色谱法测定了几种共培养物和纯培养的产烷醇脱硫弧菌膜磷脂脂肪酸（PLFA）。与脱硫弧菌共培养的产烷醇脱硫弧菌含有两种生物的 PLFA，而与产甲烷八叠球菌共培养的 PLFA 则几乎没有生物量可供分析。在 Crotonate 上生长的纯培养产烷醇脱硫弧菌为分析 PLFA 提供了最佳材料。产烷醇脱硫弧菌的主要 PLFA 是单不饱和的 16:1omega7c 和 16:1omega9c 以及饱和的 16:0 和 14:0。检测到低浓度的二不饱和 18:2omega6。PLFA 分析为确定从厌氧环境中获得的 PLFA 图谱提供了额外的信息。此外，这些信息可能有助于理解产烷醇脱硫弧菌和其他共生细菌的生理学和系统发育。
