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6
Underestimation of DNA synthesis by [h]thymidine incorporation in marine bacteria.海洋细菌中[h]胸苷掺入法低估 DNA 合成。
Appl Environ Microbiol. 1988 Dec;54(12):3165-8. doi: 10.1128/aem.54.12.3165-3168.1988.

本文引用的文献

1
Further Verification of the Isotope Dilution Approach for Estimating the Degree of Participation of [H]thymidine in DNA Synthesis in Studies of Aquatic Bacterial Production.进一步验证同位素稀释法估算[H]胸腺嘧啶核苷参与水生细菌生产力研究中 DNA 合成的程度。
Appl Environ Microbiol. 1986 Nov;52(5):1212-4. doi: 10.1128/aem.52.5.1212-1214.1986.
2
Impact of storms on heterotrophic activity of epilimnetic bacteria in a southwestern reservoir.风暴对西南水库表水层异养细菌活性的影响。
Appl Environ Microbiol. 1986 Jun;51(6):1259-63. doi: 10.1128/aem.51.6.1259-1263.1986.
3
Measurements of diel rates of bacterial secondary production in aquatic environments.水生环境中细菌次级生产力昼夜变化率的测量。
Appl Environ Microbiol. 1984 Apr;47(4):632-8. doi: 10.1128/aem.47.4.632-638.1984.
4
Estimating Bacterioplankton Production by Measuring [H]thymidine Incorporation in a Eutrophic Swedish Lake.用 [H]胸腺嘧啶核苷掺入法估算富营养化瑞典湖泊中的细菌浮游生物的产量。
Appl Environ Microbiol. 1983 Jun;45(6):1709-21. doi: 10.1128/aem.45.6.1709-1721.1983.
5
Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california.不列颠哥伦比亚省、南极洲和加利福尼亚州沿海海域的细菌浮游生物次级生产力估计。
Appl Environ Microbiol. 1980 Jun;39(6):1085-95. doi: 10.1128/aem.39.6.1085-1095.1980.
6
Validity of the tritiated thymidine method for estimating bacterial growth rates: measurement of isotope dilution during DNA synthesis.用于估计细菌生长速率的氚化胸腺嘧啶核苷方法的有效性：DNA合成过程中同位素稀释的测量
Appl Environ Microbiol. 1984 Dec;48(6):1076-83. doi: 10.1128/aem.48.6.1076-1083.1984.
7
Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats.水生和陆地生境中非光合微生物原位活性的测定。
Annu Rev Microbiol. 1985;39:321-46. doi: 10.1146/annurev.mi.39.100185.001541.

考虑到胸苷掺入测定中的同位素稀释的后果。

Consequences of accounting for isotopic dilution in thymidine incorporation assays.

机构信息

Department of Biology, The University of Texas at Arlington, Arlington, Texas 76019.

出版信息

Appl Environ Microbiol. 1988 Jul;54(7):1868-70. doi: 10.1128/aem.54.7.1868-1870.1988.

DOI:10.1128/aem.54.7.1868-1870.1988
PMID:16347698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202761/
Abstract

Rates of thymidine incorporation into DNA were corrected for isotope dilution by internal nucleotide pools and were compared with rates obtained from uncorrected data. Differences as large as 109% were observed between corrected and uncorrected estimates of thymidine incorporation. The degree of underestimation varied seasonally and, to a lesser extent, spatially.

摘要

胸腺嘧啶核苷掺入 DNA 的速率通过内部核苷酸池进行同位素稀释校正，并与未经校正的数据获得的速率进行比较。校正和未校正的胸腺嘧啶核苷掺入估计值之间的差异高达 109%。低估的程度随季节而变化，在较小程度上也随空间而变化。