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采用气液色谱法测定活性污泥中的聚-β-羟基丁酸酯和聚-β-羟基戊酸酯。

Determination of Poly-beta-Hydroxybutyrate and Poly-beta-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography.

机构信息

Environmental Engineering Group, Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, Canada V6T 1W5.

出版信息

Appl Environ Microbiol. 1988 Sep;54(9):2325-7. doi: 10.1128/aem.54.9.2325-2327.1988.

DOI:10.1128/aem.54.9.2325-2327.1988
PMID:16347745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202859/
Abstract

A convenient gas-liquid chromatography procedure to quantify poly-beta-hydroxybutyrate and poly-beta-hydroxyvalerate in activated sludge was developed by combining lyophilization of the samples, purification of the chloroform phase by water reextraction, and the use of capillary columns. With a flame ionization detector the sensitivity was estimated at 10 g/liter.

摘要

建立了一种简便的气-液色谱法，可用于定量测定活性污泥中的聚-β-羟基丁酸和聚-β-羟基戊酸。该法将样品冻干，用氯仿提取，水反萃取净化，然后用毛细管柱进行分析。采用火焰离子化检测器，其检测限可达 10g/L。

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本文引用的文献

1
Combined Determination of Poly-beta-Hydroxyalkanoic and Cellular Fatty Acids in Starved Marine Bacteria and Sewage Sludge by Gas Chromatography with Flame Ionization or Mass Spectrometry Detection.采用火焰离子化或质谱检测气相色谱法联合测定饥饿海洋细菌和污水污泥中的聚β-羟基烷酸和细胞脂肪酸。
Appl Environ Microbiol. 1986 Oct;52(4):905-10. doi: 10.1128/aem.52.4.905-910.1986.
2
Polymeric Beta-Hydroxyalkanoates from Environmental Samples and Bacillus megaterium.从环境样本和巨大芽孢杆菌中提取的聚合β-羟基烷酸酯。
Appl Environ Microbiol. 1983 Jan;45(1):71-8. doi: 10.1128/aem.45.1.71-78.1983.
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Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide.在溴化钠中用密度梯度离心法纯化聚-β-羟基丁酸酯。
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Assay of poly-beta-hydroxybutyric acid.聚-β-羟基丁酸的测定
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The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species.芽孢杆菌属中聚-β-羟基丁酸包涵体的分离与测定
J Gen Microbiol. 1958 Aug;19(1):198-209. doi: 10.1099/00221287-19-1-198.
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The role and regulation of energy reserve polymers in micro-organisms.能量储备聚合物在微生物中的作用与调控
Adv Microb Physiol. 1973;10:135-266. doi: 10.1016/s0065-2911(08)60088-0.
7
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