Department of Biology, Memphis State University, Memphis, Tennessee 38152.
Appl Environ Microbiol. 1989 Jan;55(1):266-7. doi: 10.1128/aem.55.1.266-267.1989.
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 mug of kinetin per liter.
维氏固氮菌 OP 在限定培养基中生长至静止期。通过 XAD-2 和 Sephadex LH-20 色谱、薄层层析、烟草愈伤组织生物测定和酶免疫测定对无细胞培养液中的细胞分裂素含量进行了分析。检测到三个细胞分裂素活性级分,并初步鉴定为反式玉米素、异戊烯基腺苷和异戊烯基腺嘌呤。总细胞分裂素活性相当于每升 0.75 微克激动素。