Developmental plasticity of in response to P deficiency: Modulation by inoculation with phosphate-solubilizing bacteria.

BACKGROUND

Mineral phosphorus (P) fertilizers must be used wisely in order to preserve rock phosphate, a limited and non-renewable resource. The use of bio-inoculants to improve soil nutrient availability and trigger an efficient plant response to nutrient deficiency is one potential strategy in the attempt to decrease P inputs in agriculture.

METHOD

An in vitro co-cultivation system was used to study the response of to contrasted P supplies (soluble and poorly soluble forms of P) and inoculation with P solubilizing bacteria. 's responses to P conditions and inoculation with bacteria were studied in terms of developmental plasticity and P use efficiency.

RESULTS

showed plasticity in its biomass allocation pattern in response to variable P conditions, specifically by prioritizing root development over shoot productivity under poorly soluble P conditions. Despite the ability of the bacteria to solubilize P, shoot productivity was depressed in plants inoculated with bacteria, although the root system development was maintained. The negative impact of bacteria on biomass production in might be attributed to inadequate C supply to bacteria, an increased competition for P between both organisms under P-limiting conditions, or an accumulation of toxic bacterial metabolites in our cultivation system. Both P and inoculation treatments impacted root system morphology. The modulation of 's developmental response to P supplies by P solubilizing bacteria did not lead to improved P use efficiency.

CONCLUSION

Our results support the hypothesis that plastic responses of cultivated under P-limited conditions are modulated by P solubilizing bacteria. The considered experimental context impacts plant-bacteria interactions. Choosing experimental conditions as close as possible to real ones is important in the selection of P solubilizing bacteria. Both persistent homology and allometric analyses proved to be useful tools that should be considered when studying the impact of bio-inoculants on plant development in response to varying nutritional context.