Department of Soil Science, University of Florida, Gainesville, Florida 32611-0151.
Appl Environ Microbiol. 1990 Jul;56(7):2073-9. doi: 10.1128/aem.56.7.2073-2079.1990.
Plants must be established quickly on replenished beaches in order to stabilize the sand and begin the dune-building process. The objective of this research was to determine whether inoculation of sea oats (Uniola paniculata L.) with bacteria (indigenous rhizosphere bacteria and N(2) fixers) alone or in combination with vesicular-arbuscular mycorrhizal fungi would enhance plant growth in beach sand. At two fertilizer-N levels, Klebsiella pneumoniae and two Azospirillum spp. did not provide the plants with fixed atmospheric N; however, K. pneumoniae increased root and shoot growth. When a sparingly soluble P source (CaHPO(4)) was added to two sands, K. pneumoniae increased plant growth in sand with a high P content. The phosphorus content of shoots was not affected by bacterial inoculation, indicating that a mechanism other than bacterially enhanced P availability to plants was responsible for the growth increases. When sea oats were inoculated with either K. pneumoniae or Acaligenes denitrificans and a mixed Glomus inoculum, there was no consistent evidence of a synergistic effect on plant growth. Nonetheless, bacterial inoculation increased root colonization by vesicular-arbuscular mycorrhizal fungi when the fungal inoculum consisted of colonized roots but had no effect on colonization when the inoculum consisted of spores alone. K. pneumoniae was found to increase spore germination and hyphal growth of Glomus deserticola compared with the control. The use of bacterial inoculants to enhance establishment of pioneer dune plants warrants further study.
为了稳定沙子并开始沙丘建造过程，必须在补充的海滩上快速建立植物。本研究的目的是确定是否可以单独或组合接种海洋燕麦（Uniola paniculata L.）细菌（本土根际细菌和 N2 固定剂）或丛枝菌根真菌来促进海滩沙中植物的生长。在两种肥料氮水平下，肺炎克雷伯氏菌和两种固氮菌不能为植物提供固定的大气氮；然而，肺炎克雷伯氏菌增加了根和茎的生长。当将一种难溶性磷源（CaHPO4）添加到两种沙中时，肺炎克雷伯氏菌增加了高磷含量沙中植物的生长。细菌接种不会影响植物的磷含量，这表明植物生长的增加不是由于细菌增强了植物对磷的利用。当海洋燕麦接种肺炎克雷伯氏菌或节杆菌属脱氮菌和混合丛枝菌根真菌接种物时，没有一致的证据表明对植物生长有协同作用。尽管如此，当接种物由单独的孢子组成时，细菌接种物增加了丛枝菌根真菌对根的定殖，但对定殖没有影响。与对照相比，肺炎克雷伯氏菌被发现增加了 Glomus deserticola 的孢子萌发和菌丝生长。使用细菌接种剂来促进先锋沙丘植物的建立值得进一步研究。
