一株阴沟肠杆菌在厌氧条件下还原六价铬的分离及特性研究。
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions.
机构信息
Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, and Department of Microbiology, Tokyo College of Pharmacy, Tokyo 192-03, Japan.
出版信息
Appl Environ Microbiol. 1989 Jul;55(7):1665-9. doi: 10.1128/aem.55.7.1665-1669.1989.
An Enterobacter cloacae strain (HO1) capable of reducing hexavalent chromium (chromate) was isolated from activated sludge. This bacterium was resistant to chromate under both aerobic and anaerobic conditions. Only the anaerobic culture of the E. cloacae isolate showed chromate reduction. In the anaerobic culture, yellow turned white with chromate and the turbidity increased as the reduction proceeded, suggesting that insoluble chromium hydroxide was formed. E. cloacae is likely to utilize toxic chromate as an electron acceptor anaerobically because (i) the anaerobic growth of E. cloacae HO1 accompanied the decrease of toxic chromate in culture medium, (ii) the chromate-reducing activity was rapidly inhibited by oxygen, and (iii) the reduction occurred more rapidly in glycerol- or acetate-grown cells than in glucose-grown cells. The chromate reduction in E. cloacae HO1 was observed at pH 6.0 to 8.5 (optimum pH, 7.0) and at 10 to 40 degrees C (optimum, 30 degrees C).
从活性污泥中分离到一株能够还原六价铬（铬酸盐）的阴沟肠杆菌（HO1）菌株。该细菌在需氧和厌氧条件下均对铬酸盐具有抗性。只有阴沟肠杆菌分离物的厌氧培养显示出铬酸盐还原。在厌氧培养中，随着还原的进行，铬酸盐使黄色变为白色，浊度增加，表明形成了不溶性氢氧化铬。阴沟肠杆菌很可能利用有毒的铬酸盐作为电子受体进行厌氧呼吸，因为：（i）阴沟肠杆菌 HO1 的厌氧生长伴随着培养基中有毒铬酸盐的减少；（ii）氧迅速抑制了铬酸盐还原活性；（iii）在甘油或乙酸盐生长的细胞中比在葡萄糖生长的细胞中还原发生得更快。在 pH 值为 6.0 到 8.5（最佳 pH 值为 7.0）和 10 到 40 摄氏度（最佳温度为 30 摄氏度）的条件下观察到阴沟肠杆菌 HO1 中的铬酸盐还原。
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