Bopp L H, Chakrabarty A M, Ehrlich H L
J Bacteriol. 1983 Sep;155(3):1105-9. doi: 10.1128/jb.155.3.1105-1109.1983.
Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified. Loss of the plasmid (pLHB1) by spontaneous segregation or mitomycin C curing resulted in a simultaneous loss of chromate resistance. Subsequent transformation of such strains with purified pLHB1 plasmid DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and the plasmid. When pLHB1 was transferred by conjugation to Escherichia coli, the plasmid still conferred chromate resistance.
从哈得逊河上游受铬污染沉积物中分离出的荧光假单胞菌LB300的耐铬性被发现是由质粒决定的。通过自发分离或丝裂霉素C消除而导致质粒（pLHB1）丢失，会同时导致耐铬性丧失。随后用纯化的pLHB1质粒DNA对这类菌株进行转化，会同时重新获得耐铬性表型和该质粒。当pLHB1通过接合转移到大肠杆菌时，该质粒仍能赋予耐铬性。
