Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géodésie, Université Laval, Sainte-Foy, Quebec, Canada G1K 7P4.
Appl Environ Microbiol. 1989 Sep;55(9):2320-5. doi: 10.1128/aem.55.9.2320-2325.1989.
Transformed roots of carrot were used to determine the effects of root metabolites on hyphal development from spores of the vesicular-arbuscular mycorrhizal fungus Gigaspora margarita. Hyphal growth of this obligately biotrophic symbiont was greatly stimulated by a synergistic interaction between volatile and exudated factors produced by roots. Root volatiles alone provided little stimulation, and root exudates alone had no effect. For the first time, carbon dioxide was demonstrated to be a critical root volatile involved in the enhancement of hyphal growth. C-labeled root volatiles were fixed by the fungus and thus strongly suggested that CO(2) served as an essential carbon source.
胡萝卜的转化根被用来确定根代谢物对泡囊丛枝菌属菌（Gigaspora margarita）孢子菌丝发育的影响。这种专性共生体的菌丝生长受到根分泌的挥发物和渗出物协同作用的极大刺激。单独的根挥发物几乎没有刺激作用，单独的根渗出物也没有效果。首次证明二氧化碳是参与增强菌丝生长的关键根挥发物。真菌固定了 14C 标记的根挥发物，因此强烈表明 CO（2）是一种必需的碳源。
