Laboratoire de Botanique, Cryptogamie, Biologie Cellulaire et Génétique, 38243 Meylan, France, and Department of Biochemistry, The University of Georgia, Athens, Georgia 30602.
Appl Environ Microbiol. 1989 Sep;55(9):2391-8. doi: 10.1128/aem.55.9.2391-2398.1989.
Ferulic acid metabolism was studied in cultures of two micromycetes producing different amounts of phenol oxidases. In cultures of the low phenol oxidase producer Paecilomyces variotii, ferulic acid was decarboxylated to 4-vinylguaiacol, which was converted to vanillin and then either oxidized to vanillic acid or reduced to vanillyl alcohol. Vanillic acid underwent simultaneously an oxidative decarboxylation to methoxyhydroquinone and a nonoxidative decarboxylation to guaiacol. Methoxyhydroquinone and guaiacol were demethylated to yield hydroxyquinol and catechol, respectively. Catechol was hydroxylated to pyrogallol. Degradation of ferulic acid by Paecilomyces variotii proceeded mainly via methoxyhydroquinone. The high phenol oxidase producer Pestalotia palmarum catabolized ferulic acid via 4-vinylguaiacol, vanillin, vanillyl alcohol, vanillic acid, and methoxyhydroquinone. However, the main reactions observed with this fungus involved polymerization reactions.
研究了两种产不同量酚氧化酶的真菌培养物中的阿魏酸代谢。在低酚氧化酶产生菌拟青霉的培养物中，阿魏酸脱羧生成 4-乙烯基愈创木酚，然后转化为香草醛，再被氧化成香草酸或还原为香草醇。香草酸同时发生氧化脱羧生成甲氧基对苯二酚和非氧化脱羧生成愈创木酚。甲氧基对苯二酚和愈创木酚分别脱甲基生成对苯二酚和邻苯二酚。邻苯二酚被羟化生成焦儿茶酚。拟青霉主要通过甲氧基对苯二酚降解阿魏酸。高酚氧化酶产生菌棕榈拟盘多毛孢通过 4-乙烯基愈创木酚、香草醛、香草醇、香草酸和甲氧基对苯二酚代谢阿魏酸。然而，该真菌的主要反应涉及聚合反应。
